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isca 8×60k v2 array  (Agilent technologies)


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    Structured Review

    Agilent technologies isca 8×60k v2 array
    Isca 8×60k V2 Array, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isca+array/bio_rxiv__2024__02__08__579413-172-12-11
    Average 90 stars, based on 1 article reviews
    isca 8×60k v2 array - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Hereditary multiple exostoses caused by a chromosomal inversion removing part of EXT1 gene
    Article Snippet: DNA samples of the patient and both biological parents were obtained by isolation from 2 ml peripheral blood using the Qiagen Midi Kit according to the manufacturer’s protocol (Qiagen, Valencia, CA). .. Array-based Comparative Genomic Hybridization (array CGH) was performed using SurePrint ISCA array (Agilent Technologies Inc., Santa Clara) with 60,000 oligos according to the manufacturer’s recommendations. .. The array was scanned at 2 mm resolution using the Agilent DNA microarray scanner and fluorescent ratios were calculated using the Agilent Cytogenomics-version 5.1 (Agilent Technologies Inc., Santa Clara).

    Article Title: Expanding the phenotype and genotype in Thauvin-Robinet-Faivre syndrome: A new patient with a novel variant and additional clinical findings.
    Article Snippet: Correspondence Ferda E. Percin, Faculty of Medicine, Department of Medical Genetics, Gazi University, Emniyet District, Mevlana Boulevard, No: 29, 06560, Yenimahalle/Ankara, Turkey.. Email: ferdaep@yahoo.com Abstract Thauvin-Robinet-Faivre syndrome (TROFAS; OMIM #617107) is a rare autosomal recessive overgrowth syndrome characterized by generalized overgrowth, dysmorphic facial features, and delayed psychomotor development caused by biallelic pathogenic variants in the FGF-1 intracellular binding protein (FIBP) gene.. To date, only four patients from two families have been reported.

    Article Title: CHD2 pathogenic nonsense variant in a three-generation family with variable phenotype and a paracentric inversion 16: Case report
    Article Snippet: Evaluation was done on a Axioplan microscope equipped with a suited software (ISIS, MetaSystems, Altlussheim, Germany). .. Array-based Comparative Genomic Hybridization was performed using SurePrint ISCA array (Agilent-version 2.0) with 60,000 oligos using build GRCh37 (hg19). .. Library preparation for CES was performed for the family trio, using the TruSight One sequencing panel (Illumina, San Diego, CA, USA; part number FC-141-1007) according to the manufacturer's protocol.

    Microarray:

    Article Title: Two novel variations p.(Ser1275Thr) and p.(Ser1275Arg) in FLT4 causing prenatal hereditary lymphedema type 1.
    Article Snippet: .. Fetal CGH array (Agilent Whole Genome ISCA 8 60k microarray) performed on amniocentesis ruled out the presence of a copy number variant as a cause of the phenotype. ..

    Variant Assay:

    Article Title: Two novel variations p.(Ser1275Thr) and p.(Ser1275Arg) in FLT4 causing prenatal hereditary lymphedema type 1.
    Article Snippet: .. Fetal CGH array (Agilent Whole Genome ISCA 8 60k microarray) performed on amniocentesis ruled out the presence of a copy number variant as a cause of the phenotype. ..

    Labeling:

    Article Title: The challenges in the interpretation of genetic variants detected by genomics techniques in patients with congenital anomalies
    Article Snippet: Patient and control DNA samples were cut into fragments and then labeled with cyanine 3 or cyanine 5 dyes using Agilent's sure tag DNA Labeling Kit (Agilent Technologies). .. The labeled samples were mixed in equal proportions and hybridized using a SurePrint G3 ISCA v2 8X60K array (Agilent Technologies, Inc) according to the manufacturer's protocol. .. Microarrays were scanned using a dual‐laser scanner (INNOPSYS ‐ InnoScan Microarray Scanners‐InnoScan 900).

    Article Title: The challenges in the interpretation of genetic variants detected by genomics techniques in patients with congenital anomalies.
    Article Snippet: DNA Blood and Tissue kit (Omega Bio- Tek) following the manufacturer's protocol. .. Patient and control DNA samples were cut into fragments and then labeled with cyanine 3 or cyanine 5 dyes using Agilent's sure tag DNA Labeling Kit (Agilent Technologies).9 The labeled samples were mixed in equal proportions and hybridized using a SurePrint G3 ISCA v2 8X60K array (Agilent Technologies, Inc) according to the manufacturer's protocol. .. Microarrays were scanned using a dual- laser scanner (INNOPSYS - InnoScan Microarray Scanners- InnoScan 900).

    other:

    Article Title: Copy-number variants in the ACMG secondary finding genes: A reporting framework for clinical cytogeneticists
    Article Snippet: DNA from peripheral blood specimens were analyzed for CNVs by array comparative genomic hybridization using either a 135K CGH (Roche NimbleGen) or a 180K CGH + SNP oligonucleotide array (ISCA design, Agilent), as previously described.14 Patients’ clinical presentations were obtained from a retrospective medical chart review.

    Article Title: Decoding morphogen patterning of human neural organoids with a multiplexed single-cell transcriptomic screen
    Article Snippet: All lines were tested regularly for copy number changes using the Agilent ISCA 8×60K v2 array and no abnormalities were detected.

    Control:

    Article Title: The challenges in the interpretation of genetic variants detected by genomics techniques in patients with congenital anomalies.
    Article Snippet: DNA Blood and Tissue kit (Omega Bio- Tek) following the manufacturer's protocol. .. Patient and control DNA samples were cut into fragments and then labeled with cyanine 3 or cyanine 5 dyes using Agilent's sure tag DNA Labeling Kit (Agilent Technologies).9 The labeled samples were mixed in equal proportions and hybridized using a SurePrint G3 ISCA v2 8X60K array (Agilent Technologies, Inc) according to the manufacturer's protocol. .. Microarrays were scanned using a dual- laser scanner (INNOPSYS - InnoScan Microarray Scanners- InnoScan 900).

    DNA Labeling:

    Article Title: The challenges in the interpretation of genetic variants detected by genomics techniques in patients with congenital anomalies.
    Article Snippet: DNA Blood and Tissue kit (Omega Bio- Tek) following the manufacturer's protocol. .. Patient and control DNA samples were cut into fragments and then labeled with cyanine 3 or cyanine 5 dyes using Agilent's sure tag DNA Labeling Kit (Agilent Technologies).9 The labeled samples were mixed in equal proportions and hybridized using a SurePrint G3 ISCA v2 8X60K array (Agilent Technologies, Inc) according to the manufacturer's protocol. .. Microarrays were scanned using a dual- laser scanner (INNOPSYS - InnoScan Microarray Scanners- InnoScan 900).



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